#Super engineering calculator software#
#Super engineering calculator free#

If necessary, use a temperature gradient to further optimize and empirically determine the ideal annealing temperature for each template-primer pair combination. T m values, annealing temperature, and other data are automatically generated. To use the calculator select your DNA polymerase, type in or paste your primer sequences, and provide your final primer concentration.

The modified Breslauer's thermodynamics method (2) is is used for Tm and annealing temperature calculation of reactions with Phusion or Phire DNA Polymerases.Ī separate method is used for T m and annealing temperature calculation of reactions with Taq-based DNA polymerases. The parameters were adjusted on a set of primers seeking to maximize specificity and retain high yield with Platinum SuperFi DNA Polymerase. The modified Allawi & SantaLucia's thermodynamics method (1) is used for T m and annealing temperature calculation of reactions with Platinum SuperFi DNA Polymerase. The application is designed to calculate T m according to three different methods. Thermodynamics and NMR of internal G-T mismatches in DNA. The annealing temperature gradient should start with temperature 6–10 ☌ lower than annealing temperature generated by the calculator and increased up to the extension temperature ( two-step PCR).

To use this calculator select your DNA polymerase, type in or paste your primer sequences, and provide your final primer concentration. The parameters were adjusted on a set of primers seeking to maximize specificity and retain high yields. The modified Allawi & SantaLucia's thermodynamics method is used for T m and annealing temperature calculation of reactions with Platinum SuperFi, Phusion and Phire DNA Polymerases. The calculator also calculates the primer length, percentage of GC content, molecular weight, and extinction coefficient. Miscellaneous enginering calculators and reference tables.The calculator calculates recommended T m (melting temperature) of primers and PCR annealing temperature based on the primer pair sequence, primer concentration, and DNA polymerase used in PCR. Online connection calculator for Hollow Structural Sections in structural design. Contains a substantial collection of online calculators related to aerodynamics, acoustics, civil engineering, electronics, mathematics, mechanical engineering, vibration, optics, physics & signal processing. Also includes Engineering Design Aids and a Unit Conversion Calculator Includes a list of calculators by categories of finance, design, mechanics, and mathematics. The website features hundreds of calculators for solving equations and formulas in field of Electrical, Mechanical, Chemical, Electronics, Civil, Metallurgy, Oil & Gas, Optical, Plastics, Ceramics, Physics, Maths and more. Convert from just about any imaginable unit. Developed and maintained by MAYA Heat Transfer Technologies.

#Super engineering calculator free#

Free online calculators that solve common heat transfer problems. The site is free to use and provides data on most steels and aluminum alloys.

#Super engineering calculator software#

This website contains a useful set of web-based fatigue analysis software tools for computing fatigue lives of metallic machine components and structures.